Assessment of FadV-9 based vectored vaccine expressing NetB antigen in protection against necrotic enteritis in chickens
Principal Investigator
Éva Nagy, University of Guelph
Year the work was done
2018-2020
Overall objective
- Analysis of fowl adenovirus 9 based recombinants containing the netB gene of Campylobacter perfringens (in vitro studies, and evaluation of the recombinants by immunization and challenge studies of chickens (in vivo studies).
Outcomes
- The research team generated and characterized two fowl adenovirus 9 (FAdV-9) based recombinant viruses expressing the netB gene of Clostridium perfringens, the more likely causative agent of necrotic enteritis of chickens.
- It was shown that FAdV-9 is a suitable vector for virus antigen gene delivery (e.g. influenza virus) but seems that it is not a suitable vector for C. perfringens toxin gene delivery.
- The research demonstrated that the very left end of FAdV-9 genome (nts 73-574) has promoter function, which provides an improved platform for multivalent vaccine development.
Application
- Fowl adenovirus (FAdV-9 in particular) is an excellent vector for delivering viral genes for immunization of poultry. However, it will not be a suitable vector to be used against necrotic enteritis.
This research applies to
Breeders, Broilers, Hatcheries, Layers