Antimicrobials not antibiotic based platform to treat Salmonella enteriditis infection in laying hens


Principal Investigator

Marya Ahmed, University of Prince Edward Island

Year the work was done

2018-2021

Overall objective

  • To prepare vitamin B5 functionalized cationic amphipathic peptides of different hydrophobicity and cationic character by solid phase peptide synthesis and study their biocompatibility and antimicrobial properties in gram positive and gram negative bacteria in vitro.

Outcomes

  • Two peptides presented potent antibacterial efficacies against pathogenic bacteria, including Salmonella.
  • AMPs (antimicrobial peptides) showed antibacterial activity against both gram-positive and gram-negative bacteria.
  • AMPs displayed anti-inflammatory properties by suppressing pro-inflammatory cytokine production in cells challenged by LPS (lipopolysaccharide).

Application

  • The major challenge to the clinical application of synthetic AMPs is their high cost of production. However, with further advances in peptide synthesis technology, synthetic AMPs may present a promising alternative to many traditional antimicrobial agents.
  • For long-term objectives, the research team will focus on producing peptides to treat S. enteriditis infection in poultry.
  • This research applies to

    Layers, Processors

This research was supported by Agriculture and Agri-Food Canada, Canadian Poultry Research Council, Egg Farmers of Canada and Biovectra.