Assessment of FadV-9 based vectored vaccine expressing NetB antigen in protection against necrotic enteritis in chickens


Principal Investigator

Éva Nagy, University of Guelph

Year the work was done

2018-2020

Overall objective

  • Analyze fowl adenovirus 9 based recombinants containing the NetB gene of Campylobacter perfringens (in vitro studies) and evaluate the recombinants by immunization and challenge studies of chickens (in vivo studies).

Outcomes

  • The research team generated and characterized two fowl adenovirus 9 (FAdV-9) based recombinant viruses expressing the NetB gene of Clostridium perfringens, the more likely causative agent of necrotic enteritis of chickens.
  • It was shown that FAdV-9 is a suitable vector for virus antigen gene delivery (e.g. influenza virus) but seems that it is not a suitable vector for C. perfringens toxin gene delivery.
  • The research demonstrated that the very left end of FAdV-9 genome (nts 73-574) has promoter function, which provides an improved platform for multivalent vaccine development.

Application

  • Fowl adenovirus (FAdV-9 in particular) is an excellent vector for delivering viral genes for immunization of poultry. However, it will not be a suitable vector to be used against necrotic enteritis.

This research applies to

Breeders, Broilers, Hatcheries, Layers

This research was supported by Agriculture and Agri-Food Canada, Canadian Poultry Research Council, National Sciences and Engineering Research Council of Canada, and industry.