Development of novel and rational vaccine formulations against avian influenza virus infection in chickens

Principal Investigator

Shayan Sharif, University of Guelph

Year the work was done


Overall objective

  • To investigate ways to enhance prophylactic strategies against avian influenza virus.


  • Pathogen associated molecular patterns (PAMPs) were evaluated and three PAMPs with the ability to enhance and immune capabilities were identified and used as adjuvants for an inactivated AI vaccine.
  • Vaccine formulations that included PAMP and different administration routes for the vaccine were developed.
  • An adenovirus vector for administration of the vaccine was identified, further developed, and used for AI vaccination.


  • A virus vector that can be used for delivery avian influenza virus as well as potentially other avian viruses was developed. This vector has been licensed and is being commercialized.
  • Several combinations vaccine adjuvants in chickens have been identified and have the potential for commercialization.

This research applies to

Breeders, Broilers, Layers, Turkeys

This research was supported by Agriculture and Agri-Food Canada, the Ontario Ministry of Agriculture, Food and Rural Affairs, and Canadian Poultry Research Council.